@article{330,

title = {Role of neurogenic genes in establishment of follicle cell fate and oocyte polarity during oogenesis in Drosophila.},

author = { H Ruohola and K A Bremer and D Baker and J R Swedlow and L Y Jan and Y N Jan},

issn = {0092-8674},

year  = {1991},

date = {1991-08-01},

journal = {Cell},

volume = {66},

pages = {433-49},

abstract = {Oogenesis in Drosophila involves specification of both germ cells and the surrounding somatic follicle cells, as well as the determination of oocyte polarity. We found that two neurogenic genes, Notch and Delta, are required in oogenesis. These genes encode membrane proteins with epidermal growth factor repeats and are essential in the decision of an embryonic ectodermal cell to take on the fate of neuroblast or epidermoblast. In oogenesis, mutation in either gene leads to an excess of posterior follicle cells, a cell fate change reminiscent of the hyperplasia of neuroblasts seen in neurogenic mutant embryos. Furthermore, the Notch mutation in somatic cells causes mislocalization of bicoid in the oocyte. These results suggest that the neurogenic genes Notch and Delta are involved in both follicle cell development and the establishment of anterior-posterior polarity in the oocyte.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{332,

title = {Reconstitution of SEC gene product-dependent intercompartmental protein transport},

author = { D Baker and L Hicke and M Rexach and M Schleyer and R Schekman},

url = {https://www.bakerlab.org/wp-content/uploads/2016/06/baker88.pdf},

issn = {0092-8674},

year  = {1988},

date = {1988-07-01},

journal = {Cell},

volume = {54},

pages = {335-44},

abstract = {Transport of alpha-factor precursor from the endoplasmic reticulum to the Golgi apparatus has been reconstituted in gently lysed yeast spheroplasts. Transport is measured through the coupled addition of outer-chain carbohydrate to [35S]methionine-labeled alpha-factor precursor translocated into the endoplasmic reticulum of broken spheroplasts. The reaction is absolutely dependent on ATP, stimulated 6-fold by cytosol, and occurs between physically separable sealed compartments. Transport is inhibited by the guanine nucleotide analog GTP gamma S. sec23 mutant cells have a temperature-sensitive defect in endoplasmic reticulum-to-Golgi transport in vivo. This defect has been reproduced in vitro using sec23 membranes and cytosol. Transport at 30 degrees C with sec23 membranes requires addition of cytosol containing the SEC23 (wild-type) gene product. This demonstrates that an in vitro inter-organelle transport reaction depends on a factor required for transport in vivo. Complementation of sec mutations in vitro provides a functional assay for the purification of individual intercompartmental transport factors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}