Home » Trapping a transition state in a computationally designed protein bottle

Trapping a transition state in a computationally designed protein bottle

Mills_graphical_abstract

The fleeting lifetimes of the transition states (TSs) of chemical reactions make determination of their three-dimensional structures by diffraction methods a challenge. Here we used packing interactions within the core of a protein to stabilize the planar TS conformation for rotation around the central carbon-carbon bond of biphenyl so that it could be directly observed by x-ray crystallography. The computational protein design software Rosetta was used to design a pocket within threonyl-transfer RNA synthetase from the thermophile Pyrococcuss abyssi that forms complementary Van der Waals interactions with a planar biphenyl. This latter moiety was introduced biosynthetically as the side chain of the non-canonical amino acid p-biphenylalanine. Through iterative rounds of computational design and structural analysis, we identified a protein in which the side chain of-biphenylalanine is trapped in the energetically disfavored, coplanar conformation of the TS of the bond rotation reaction.

Pearson, A.D., Mills, J.H. et al. Science 347(6224), 863-7