A "loop entropy reduction" phage-display selection for folded amino acid sequences

TitleA "loop entropy reduction" phage-display selection for folded amino acid sequences
Publication TypeJournal Article
Year of Publication2001
AuthorsMinard, P., Scalley-Kim M., Watters A., & Baker D.
JournalProtein science
Volume10
Issue1
Pagination129-34
Date Published2001 Jan
ISSN0961-8368
KeywordsAmino Acid Sequence, Entropy, Evolution, Molecular, Models, Molecular, Peptide Library, Primary Publication, Protein Denaturation, Protein Folding, Recombinant Fusion Proteins
Abstract

As a step toward selecting folded proteins from libraries of randomized sequences, we have designed a 'loop entropy reduction'-based phage-display method. The basic premise is that insertion of a long disordered sequence into a loop of a host protein will substantially destabilize the host because of the entropic cost of closing a loop in a disordered chain. If the inserted sequence spontaneously folds into a stable structure with the N and C termini close in space, however, this entropic cost is diminished. The host protein function can, therefore, be used to select folded inserted sequences without relying on specific properties of the inserted sequence. This principle is tested using the IgG binding domain of protein L and the lck SH2 domain as host proteins. The results indicate that the loop entropy reduction screen is capable of discriminating folded from unfolded sequences when the proper host protein and insertion point are chosen.

Alternate JournalProtein Sci.
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