Atomic model of the type III secretion system needle

TitleAtomic model of the type III secretion system needle
Publication TypeManuscript
Year of Publication2012
AuthorsLoquet, A., Sgourakis N. G., Gupta R., Giller K., Riedel D., Goosmann C., Griesinger C., Kolbe M., Baker D., Becker S., & Lange A.
Collection TitleNature
KeywordsCollaborative Publication, Primary Publication

Pathogenic bacteria using a type III secretion system (T3SS) to manipulate host cells cause many different infections including Shigella dysentery, typhoid fever, enterohaemorrhagic colitis and bubonic plague. An essential part of the T3SS is a hollow needlelike protein filament through which effector proteins are injected into eukaryotic host cells3–6. Currently, the three-dimensional structure of the needle is unknown because it is not amenable to X-ray crystallography and solution NMR, as a result of its inherent non-crystallinity and insolubility. Cryo-electron microscopy combined with crystal or solution NMRsubunit structures has recently provided a powerful hybrid approach for studying supramolecular assemblies7–12, esulting in low-resolution and medium-resolution models13–17. However, such approaches cannot deliver atomic details, especially of the crucial subunit–subunit interfaces, because of the limited cryo-electron microscopic resolution obtained in these studies. Here we report an alternative approach combining recombinant wild-type needle production, solid-state NMR, electron microscopy and Rosetta modelling to reveal the supramolecular interfaces and ultimately the complete atomic structure of the Salmonella typhimurium T3SS needle. We show that the 80-residue subunits form a right-handed helical assembly with roughly 11 subunits per two turns, similar to that of the flagellar filament of S. typhimurium. In contrast to established models of the needle in which the amino terminus of the protein subunit was assumed to be a-helical and positioned inside the needle, our model reveals an extended amino-terminal domain that is positioned on the surface of the needle, while the highly conserved carboxy terminus points towards the lumen.

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