Crystal structure of a monomeric retroviral protease solved by protein folding game players

TitleCrystal structure of a monomeric retroviral protease solved by protein folding game players
Publication TypeJournal Article
Year of Publication2011
AuthorsKhatib, F., DiMaio F., Cooper S., Kazmierczyk M., Gilski M., Krzywda S., Zabranska H., Pichova I., Thompson J., Popović Z., Jaskolski M., & Baker D.
Corporate AuthorsContenders Group, Foldit, & Void Crushers Group, Foldit
JournalNature structural & molecular biology
Volume18
Issue10
Pagination1175-7
Date Published2011 Oct
ISSN1545-9985
KeywordsCrystallography, X-Ray, Models, Molecular, Peptide Hydrolases, Primary Publication, Protein Conformation, Protein Folding, Retroviridae, Viral Proteins
Abstract

Following the failure of a wide range of attempts to solve the crystal structure of M-PMV retroviral protease by molecular replacement, we challenged players of the protein folding game Foldit to produce accurate models of the protein. Remarkably, Foldit players were able to generate models of sufficient quality for successful molecular replacement and subsequent structure determination. The refined structure provides new insights for the design of antiretroviral drugs.

DOI10.1038/nsmb.2119
Alternate JournalNat. Struct. Mol. Biol.
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