Computational design of orthogonal nucleoside kinases

TitleComputational design of orthogonal nucleoside kinases
Publication TypeJournal Article
Year of Publication2010
AuthorsLiu, L., Murphy P., Baker D., & Lutz S.
JournalChemical communications
Volume46
Issue46
Pagination8803-5
Date Published2010 Dec 14
ISSN1364-548X
KeywordsCollaborative Publication
Abstract

We report the computational enzyme design of an orthogonal nucleoside analog kinase for 3'-deoxythymidine. The best kinase variant shows an 8500-fold change in substrate specificity, resulting from a 4.6-fold gain in catalytic efficiency for the nucleoside analog and a 2000-fold decline for the native substrate thymidine.

Alternate JournalChem. Commun. (Camb.)
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