Computational design of novel protein binders and experimental affinity maturation

TitleComputational design of novel protein binders and experimental affinity maturation
Publication TypeJournal Article
Year of Publication2013
AuthorsWhitehead, T. A., Baker D., & Fleishman S. J.
JournalMethods in enzymology
Volume523
Pagination1-19
Date Published2013
ISSN1557-7988
KeywordsCollaborative Publication
Abstract

Computational design of novel protein binders has recently emerged as a useful technique to study biomolecular recognition and generate molecules for use in biotechnology, research, and biomedicine. Current limitations in computational design methodology have led to the adoption of high-throughput screening and affinity maturation techniques to diagnose modeling inaccuracies and generate high activity binders. Here, we scrutinize this combination of computational and experimental aspects and propose areas for future methodological improvements.

DOI10.1016/B978-0-12-394292-0.00001-1
Custom1

http://www.ncbi.nlm.nih.gov/pubmed/23422423?dopt=Abstract

Alternate JournalMeth. Enzymol.
AttachmentSize
Whitehead_MethEnzymology_13V.pdf1.18 MB