Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring

TitleBioluminescent sensor proteins for point-of-care therapeutic drug monitoring
Publication TypeJournal Article
Year of Publication2014
AuthorsGriss, R., Schena A., Reymond L., Patiny L., Werner D., Tinberg C. E., Baker D., & Johnsson K.
JournalNature chemical biology
Volume10
Issue7
Pagination598-603
Date Published07/2014
ISSN1552-4469
Abstract

For many drugs, finding the balance between efficacy and toxicity requires monitoring their concentrations in the patient's blood. Quantifying drug levels at the bedside or at home would have advantages in terms of therapeutic outcome and convenience, but current techniques require the setting of a diagnostic laboratory. We have developed semisynthetic bioluminescent sensors that permit precise measurements of drug concentrations in patient samples by spotting minimal volumes on paper and recording the signal using a simple point-and-shoot camera. Our sensors have a modular design consisting of a protein-based and a synthetic part and can be engineered to selectively recognize a wide range of drugs, including immunosuppressants, antiepileptics, anticancer agents and antiarrhythmics. This low-cost point-of-care method could make therapies safer, increase the convenience of doctors and patients and make therapeutic drug monitoring available in regions with poor infrastructure.

DOI10.1038/nchembio.1554
Custom1

http://www.ncbi.nlm.nih.gov/pubmed/24907901?dopt=Abstract

Alternate JournalNat. Chem. Biol.
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